Dept. of Biotechnology Engineering
Tel : 972-8- 6477182
Fax : 972-8-64 72857
building 39 , Room 208
We develop fiber-optic as well as amperometric immunosensors to sera for pathogens such as Hepatitis C or B, West Nile virus, Ebola virus, Dengue virus, Influenza, Brucellosis, Crimean Cong Hemorragic Fever, etc...). In addition, we monitor (on-line or dispatch) environmental water toxicity such as genotoxicity, cytotoxicity, endocrine disrupting compounds, heavy metals or pathogens through fiber-optic whole-cell bioreporters, immunosensors or genosensors. We are interested in miniaturization of diagnostics to create a self-contained Biopen device.
Our work extends in studying phagocyte chemiluminescence as a disease marker for rapid diagnostics while adapting this work to phagocyte inducible cell-cultures for the study of ROS. This same method is being tested for nanotoxicity for the analysis of nanometer-scale inorganic particles. We design, synthesize, characterize a number of novel bio-receptor interfacial immobilisation reagents for interface chemistry (aptamers, dendrons, electropolymerisable monomers, photo-inducible linkers including new silanes) for use in biosensors. We create novel transducer systems for solid phase diagnostics, including ITO covered fiber optics as well as modules of integrated arrayed bacterial sensors. We are working on developing nanoscale molecular technologies such as the design and construction of a multiple-epitope phage display system, the screening for novel epitopes of Ebola virus for diagnostics, the molecular engineering of cell cultures that will luminesce in the presence of a virus (influenza, hanta, CCHF) that will be useful in drug screening,
the molecular design and validation of micro-scale pad surfaces for biochips,
and the microfluidic dispensing of reagents on a chip. In addition, we have created bacterial panels for the discovery of antibiotic –like bioactive reagents as well as the discovery of novel quorum sensing chemicals and their disruptors produced in the marine ecosystem that may be useful in improving human chemotherapy to bacterial pathogens. Work on metal enhanced bioluminescence has shown possible increase in the luminescence potential of bacterial bioreporters. We are developing various immunoassays based on magnetic bead capture of target entities which are collected by a magnet (in a liquid light guide format) or nanostructured magnetic strips for miniaturization.Our work has been spin-off in an incubator project working on affinity hydrogel formulations for their application to the reduction of nasal pollen/spore allergies. Our laboratory has received a large collaborative project on tailored nanomaterials for water management, which is located in Singapore, and covers work on monitoring pollution and bioremediation.