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1. Biosynthesis, role, and biotechnology for LC-PUFA in microalgae. 2. Genetic transformation and metabolic engineering of the model and non-model microalgae to improve biotechnology- relevant traits, elucidation of LC-PUFA and TAG metabolism mechanisms. 3. Establishing a high-confidence lipid droplet proteome of the diatom Phaeodactylum tricornutum and functional analysis of LD-associated proteins by CRISPR-CAS9 and overexpression analysis 4. Crosstalk between autophagy and lipid metabolism in microalgae 5. Application of microalgae to modulate stress and disease-resistance in fish.
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