Flow Cytometry combined with Microscopy
is an emerging field in biological research. Using cutting edge technology,
it's address the growing need for high-throughput fluorescent microscopic
detailed cellular images and morphologic information, which are useful
scientific tools for the study of cell function. However, the interpretation of
microscopic imagery can be subjective, qualitative, and laborious.
Flow cytometry is
excellent for quantitative phenotyping and yields statistically robust results by
rapidly interrogating large numbers of cells. However, flow cytometry lacks any
ability to image, so sub-cellular localization and cell function are measured
By combining the
speed, sensitivity, and phenotyping abilities of flow cytometry with the
detailed imagery and functional insight of microscopy, the ImageStreamX Mark II
overcomes the limitations of both techniques and opens the door to an extensive
range of novel applications.
Mark II is designed to be a general-purpose platform for cellular studies and
is not limited to the illustrated applications. The ImageStreamX Mark II
utilizes the same dyes and markers employed in microscopy and flow cytometry
and can perform virtually any standard flow cytometry assay with the added
value of visual confirmation.
Some featured applications
can be performed using the ImageStreamX Mark II and its powerful companion
IDEAS® image analysis software. Over 250 peer reviewed publications incorporate
IDEAS - Image
Data that is
collected by ImageStream is analyzed using IDEAS software. In IDEAS, population
statistics and imagery are completely integrated. Every dot on a scatter plot
links directly to a cell's images - click on the dot and you'll see the
corresponding cell. With its virtual sorting capability, IDEAS will show you
all the images of a cell population you define.
The IDEAS software
package is extraordinarily robust, providing hundreds of features for
every cell. IDEAS also allows you to create almost any new feature you may need
from the basic feature set.
Once you've created
an analysis scheme in IDEAS, you can save it as a template for batch processing
future experiments or to share with your colleagues.
Dr. Uzi Hadad
Building 51 , Room 325
Tel : 972-8-6428674
Fax : 972-8-6428638
Building 51 , Room 326
Tel : 972-8-6428648, 054-4660750
Email : firstname.lastname@example.org