Ilse Katz Institute for Nanoscale Science & Technology

FL920 overview

Spectrofluorimeter Overview

FLS920P Spectrofluorimeter

The FLS920P is L-geometry spectrofluorimeter for acquiring steady state luminescence

spectra, anisotropy spectra (continuous Xenon arc lamp, 450W) and the associated

fluorescence decays within a range of 230-900nm.

Signal to noise ratio of instrument 6000:1.

The instrument has different types of sample chambers: 3 position temperatures controlled

(water recirculation chiller) single position stirrer holders for liquid samples;

and front face with rotation holder for thin films, powders, slides and fibres samples.

FL920 specification

Spectrofluorimeter Specifications

FL920

System

Optical Configuration Right angle geometry

Sensitivity

Water Raman Spectrum Excitation wavelength = 350nm

Spectral bandwidth 5nm, integration time 1s

Peak Counts > 750,000cps @ 397nm

RMS Noise < 125cps @ 450nm

Signal to Noise Ratio > 6000:1

Excitation Source

Type Continuous Xenon Arc Lamp Lamp 450W, ozone free

Spectral Range 230nm – >1000nm

Monochromator

Type Czerny-Turner

Focal Length 300mm

Grating

Resolution 0.05 – 18nm

Wavelength Accuracy ± 0.2nm

Wavelength Repeatability ± 0.1nm

Minimum Step Size 0.05nm

Detector

Photomultiplier Blue Sensitive Red Sensitive

Spectral Range 200 – 680nm 200 – 870nm

Window Material UV Glass UV Glass

Dark Count Rate (+24ºC) < 100cps < 2000cps

Dark Count Rate (-20ºC) < 5cps < 50cps

Data Acquisition

Photon Counting Scalar Card Model PCS900 Plug-in PC Card

Mode of Operation Single photon counting / multi-channel scaling (FLS920-s) (FLS920-m)

Maximum Count Rate 100MHz

FL920 application examples

Spectrofluorimeter Application Examples

FL920

Figure 1: A. The fluorescence excitation and emission spectra (A) and Time-resolved fluorescence decay profile of N-naphthoquinonyl aminostilbenes (Research from the group of Prof. S. Bittner, Department of Chemistry, BGU).

Figure 2: Fluorescence emission spectra of 0.2 μM pyrene (excitation at 335 nm), normalized by the intensity of the first emission band at 373 nm, in three different solvents (water, ethanol, and tetrahydrofuran [THF]), in bacteria, and in liposomes. (Research from the group of Prof. N.Porat, Medical Department, BGU).

Figure 3: Fluorescence quenching. Fluorescence intensities of NBD-PE dye embedded within DMPC vesicles following preincubation with DAG-lactones 1–5. Initial fluorescence (at t=0, upon addition of sodium thionite) is defined as 100 %.. (Research from the group of Prof. R.Jelinek, Chemistry Department, BGU).

FL920 contact person

FL920 Contact Persons

FL920 laboratory

Dr. Sofiya Kolusheva

Ilse Katz Institute for Nanoscale Science and Technology
Ben-Gurion University of the Negev

Tel : 972-8-6428664

972-8-6428667

Fax : 972-8-6428638

Beer-Sheva 84105, Israel

Email: kolushev@gmail.com

Email: kolushev@bgu.ac.il

Fluorescence Spectroscopy

Fluorescence Spectroscopy

FL920 overview

FL920 specification

FL920 application examples

FL920 contact person