Synaptic function of synapsin
Shulman Y, Stavsky A, Fedorova T, Mikulincer D, Atias M, Radinsky I, Kahn J, Slutsky I, Gitler D (2015) ATP binding to synaspsin IIa regulates usage and clustering of vesicles in terminals of hippocampal neurons. J Neurosci 35(3):985-98. https://doi.org/10.1523/JNEUROSCI.0944-14.2015.
Tang Y, Scott D, Das U, Gitler D, Ganguly A, Roy S (2013) Fast vesicle transport is required for the slow axonal transport of synapsin. J Neurosci 33(39):15362-75. https://doi.org/10.1523/JNEUROSCI.1148-13.2013.
Vasileva M, Horstmann H, Geumann C, Gitler D, Kuner T (2012) Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission. Eur J Neurosci 36(8):3005-20. https://doi.org/10.1111/j.1460-9568.2012.08225.x.
Orenbuch A, Shalev L, Marra V, Sinai I, Lavy Y, Kahn J, Burden JJ, Staras K, Gitler D (2012) Synapsin selectively controls the mobility of resting pool vesicles at hippocampal terminals. J Neurosci 32(12):3969-80. https://doi.org/10.1523/JNEUROSCI.5058-11.2012.
Orenbuch A, Shulman Y, Lipstein N, Bechar A, Lavy Y, Brumer E, Vasileva M, Kahn J, Barki-Harrington L, Kuner T, Gitler D (2012) Inhibition of exocytosis or endocytosis blocks activity-dependent redistribution of synapsin. J Neurochem 120(2):248-58. https://doi.org/10.1111/j.1471-4159.2011.07579.x.
Synaptic function of alpha-synuclein
Atias M, Tevet Y, Sun J, Stavsky A, Tal S, Kahn J, Roy S, Gitler D (2019) Synapsins regulate α-synuclein functions. Proc Natl Acad Sci U S A 116(23):11116-11118. https://doi.org/10.1073/pnas.1903054116.
Schechter M, Atias M, Abd Elhadi S, Davidi D, Gitler D, Sharon R (2020) α-Synuclein facilitates endocytosis by elevating the steady-state levels of phosphatidylinositol 4,5-bisphosphate. J Biol Chem 295(52):18076-18090. https://doi.org/10.1074/jbc.RA120.015319.
Induction of dopaminergic neurodegeneration in an AAV model of PD
Vitic Z, Safory H, Jovanovic VM, Sarusi Y, Stavsky A, Kahn J, Kuzmina A, Toker L, Gitler D, Taube R, Friedel RH, Engelender S, Brodski C (2021) BMP5/7 protect dopaminergic neurons in an α-synuclein mouse model of Parkinson's disease. Brain 144(2):e15. https://doi.org/10.1093/brain/awaa368.
Anti synapsin auto-antibodies
Mertens R, Melchert S, Gitler D, Schou MB, Saether SG, Vaaler A, Piepgras J, Kochova E, Benfenati F, Ahnert-Hilger G, Ruprecht K, Höltje M (2018) Epitope specificity of anti-synapsin autoantibodies: Differential targeting of synapsin I domains. PLoS One 13(12):e0208636. https://doi.org/10.1371/journal.pone.0208636.
Höltje M, Mertens R, Schou MB, Saether SG, Kochova E, Jarius S, Prüss H, Komorowski L, Probst C, Paul F, Bellmann-Strobl J, Gitler D, Benfenati F, Piepgras J, Ahnert-Hilger G, Ruprecht K (2017) Synapsin-antibodies in psychiatric and neurological disorders: Prevalence and clinical findings. Brain Behav Immun 66:125-134. https://doi.org/10.1016/j.bbi.2017.07.011.
Piepgras J, Höltje M, Otto C, Harms H, Satapathy A, Cesca F, Benfenati F, Gitler D, Pich A, Zander JF, Ahnert-Hilger G, Ruprecht K (2015) Intrathecal immunoglobulin A and G antibodies to synapsin in a patient with limbic encephalitis. Neurol Neuroimmunol Neuroinflamm 2(6):e169. https://doi.org/10.1212/NXI.0000000000000169.
Diabetes-related derailment of cellular metabolism
Aghanoori MR, Margulets V, Smith DR, Kirshenbaum LA, Gitler D, Fernyhough P (2021) Sensory neurons derived from diabetic rats exhibit deficits in functional glycolysis and ATP that are ameliorated by IGF-1. Mol Metab 49:101191. https://doi.org/10.1016/j.molmet.2021.101191.
Synaptic function of NCLX
Stavsky A, Stoler O, Kostic M, Katoshevsky T, Assali EA, Savic I, Amitai Y, Prokisch H, Leiz S, Daumer-Haas C, Fleidervish I, Perrochi F, ^Gitler D, ^Sekler I (2021) Aberrant activity of mitochondrial NCLX is linked to impaired synaptic transmission and is associated with mental retardation. Commun Biol 4, 666. https://doi.org/10.1038/s42003-021-02114-0.
^ Equally contributing and corresponding authors.
Methods
Tevet Y, Gitler D (2016) Using FRAP or FRAPA to Visualize the Movement of Fluorescently Labeled Proteins or Cellular Organelles in Live Cultured Neurons Transformed with Adeno-Associated Viruses. Methods Mol Biol 1474:125-51. https://doi.org/10.1007/978-1-4939-6352-2_8.
Staras K, Mikulincer D, Gitler D (2013) Monitoring and quantifying dynamic physiological processes in live neurons using fluorescence recovery after photobleaching. J Neurochem 126(2):213-22. https://doi.org/10.1111/jnc.12240.
Levy S, Wilms CD, Brumer E, Kahn J, Pnueli L, Arava Y, Eilers J, Gitler D (2011) SpRET: highly sensitive and reliable spectral measurement of absolute FRET efficiency. Microsc Microanal 17(2):176-90. https://doi.org/10.1017/S1431927610094493.
Synaptic involvement of other proteins
Halperin D, Stavsky A, Kadir R, Drabkin M, Wormser O, Yogev Y, Dolgin V, Proskorovski-Ohayon R, Perez Y, Nudelman H, Stoler O, Rotblat B, Lifschytz T, Lotan A, Meiri G, Gitler D, Birk O (2021) CDH2 mutation affecting N-cadherin function causes attention-deficit hyperactivity disorder in humans and mice. Nature Communications. In press.
