Dr. Alon Zilka
Head of the Cytometry, Proteomic and Microscopy Unit
Ph.D.: Ben-Gurion University of the Negev, Israel
Post-doctorate: Ben-Gurion University of the Negev, Israel
Position: Research Associate
E-mail: azilha@bgu.ac.il
The service unit is equipped with high end instruments to help identify inter-molecular interactions between proteins, peptides, nucleic acids and other small molecules. The unit contains a confocal microscope and a high throughput Fluorescent/confocal microscope for quality/large scale image acquisition and analysis. Flow cell analyzers and a high speed sorter enable the analysis and sorting of cell sub populations. A Robot and a high speed plate reader provide a high throughput workbench connected to a powerful fluorescence/luminescence/absorbance analyzer.
- A laser scanning confocal microscope FV1000 equipped with 405, 488 and 543nm lasers (Olympus).
- Proteon - A protein interaction array system (Surface Plasmon Resonance) for measuring inter-
molecular affinity in a high throughput mode (Biorad)
- Monolith - A Micro Scale Thermophoresis instrument for measuring inter-molecular affinity using
capillaries to minimize the amounts of materials needed (Nanotemper).
- Operetta - A high throughput fluorescent/confocal microscope for large scale image acquisition and
statistical image analysis (Perkin Elmer)
- Synergy - A high speed cell sorter containing 2 independent sorting modules with 375, 488 and
561/594nm lasers on module 1 and 405, 488 and 640nm lasers on module 2. Each module has 6
fluorescent channels and can sort up to 4 sub populations (iCyt)
- Two Flow cytometers analyzers, one with 488 and 633nm lasers and 4 fluorescent channels
(Calibur) and another with 405 and 640nm lasers and 5 fluorescent channels an EV channel and a
sample auto loader (Eclipse) (BD and iCyt, respectively)
- A high throughput freedom Evo Robot system connected to a high speed sophisticated plate reader
(M1000) (Tecan)
