Micha Volokita.JPG

Dr. Micha Volokita

Head of the DNA Microarray and Sequencing Unit

Ph.D.: The Hebrew University of Jerusalem, Israel
Post-Doctorate: Michigan State University, USA
Position: Research Associate
DNA microarray and sequencing laboratory - Head
E-mail: volokita@bgu.ac.il

  

  • Background

The NIBN core laboratory for DNA microarrays and DNA sequencing provides a number of services that rely on high-priced instruments not usually at the daily disposal of a standard level research laboratory.

  • Services

DNA Sequencing
- Genotyping by analysis of DNA fragments
- Whole genome expression analyses
- Whole genome methylation mapping
- Whole genome mapping of cis-regulatory elements
- Single nucleotide polymorphisms (SNPs) genotyping
- Detection of unknown genetic mutations and SNPs
- Resequencing
- Tiling arrays

 

  • Equipment

- ABI Prism 3100 genetic analyzer

- Affymetrix DNA microarray platform, which includes a GeneChip Scanner 3000 7G, a Fluidics Station 
  450, a Hybridization Oven 640, and a powerful computer workstation with dual Xeon processors 
  loaded with GeneChip Operating Software (GCOS)

- Transgenomics WAVE system instruments: A denaturing high pressure liquid chromatography
   (dHPLC) machine

- 2100 Electrophoresis Bioanalyzer

  • Selected publications

Narkis G., Ofir R., Landau D., Manor E., Volokita M., Hershkowitz R., Elbedour K. and Birk O.S. (2007). Lethal Contractural Syndrome Type 3 (LCCS3) Is Caused by a Mutation in PIP5K1C, Which Encodes PIPKI gamma of the Phophatidylinsitol Pathway. Am. J. Hum. Genet. 81:530-539.

Najami N., Janda T., Barriah W., Kayam G., Tal M., Guy M. and Volokita M. (2008). Ascorbate Peroxidase Gene Family in Tomato: Its Identification and Characterization. Molec. Genet. Genomics. 279:171-182.

Volokita M., Rosilio-Brami T., Rivkin N. and Zik M. (2011). Combining comparative sequence and genomic data to ascertain phylogenetic relationships and explore the evolution of the large GDSL-lipase family in land plants. Mol. Biol. Evol. 28(1):551-565.