Zeta Potential

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Zeta Potential overview

​Equipment Overview

Zeta Potential: Laser Doppler Micro-electrophoresis

This instrument is used to measure zeta potential.

Zeta potential is measured by applying an electric field across the dispersion. Particles within the dispersion with a zeta potential will migrate toward the electrode of opposite charge with a velocity proportional to the magnitude of the zeta potential.

This velocity is measured using the technique of laser Doppler anemometry. The frequency shift or phase shift of an incident laser beam caused by these moving particles is measured as the particle mobility, and this mobility is converted to the zeta potential by inputting the dispersant viscosity, and the application of the Smoluchowski or Huckel theories. These theories are approximations useful for most applications. More recent models are available which can give a more exact conversion, but require more knowledge of the chemistry of the dispersion.

 

 



Zeta potential Specifications

Equipment Specifications (as given by Malvern)

Zeta Potential: Nano ZS

Light Source: He-Ne Laser 633nm, Max 5mW.

Measurement type: Zeta potential

Zeta potential range:  > +/-500mV

Measurement principle: Electrophoretic Light Scattering

Mobility range:  > +/- 20 ì.cm/V.s

Sensitivity: 10mg/mL (BSA).

Maximum sample concentration: 40% w/v 40% w/v

Maximum sample conductivity: 200mS/cm 200mS/cm

Signal processing: M3-PALS

Temperature control range: 0ºC - 90ºC +/-0.1.

Software:
The software is designed to be versatile and rich in features without compromising ease of use. Standard Operating Procedures (SOP) simplify routine measurements, a quality factor gives a single place for confirmation of data quality, and an expert advice system is provided to assist with data interpretation.

 

Zeta Potential application examples
Application Examples
Zeta Potential: Nano ZS
1. Evolution of the zeta-potential versus the number of layers on the GNPs

Zeta Potential of Gold nanoparticles.
(Research from the group of Prof. Yossi Kost, Department of Chem. Eng., BGU).
 
2.


In order to determine the surface charge of the polyplexes their corresponding ζ-potentials were measured. All polyplexes displayed positive ζ-potential due to an excess of the positively charged PEI. The measurements of all PICs were significantly lower than the ζ-potential observed for PEI polyplexes. These results dictate that the block copolymer micelle like particle is with PEI/siRNA or miRNA core and PEG localized in the outer shell. Thus, the targeting moieties, due to their orientation in relation to the PEI molecule, are localized on the polyplexes exterior. 
(Research from the group of Prof. Ayelet David Department of Pharmacology 
The Faculty of Health Sciences., BGU).
Zeta Potential contact person
Contact Persons
Zeta Potential: Nano ZS
Dr. Sharon Hazan (Vanounou)
Ilse Katz Institute for Nanoscale Science and Technology
Ben-Gurion University of the Negev
Tel : 972-8-6479396
Fax : 972-8-6428638
Beer-Sheva 84105, Israel
Email: sharvan@bgu.ac.il​